| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Influenza A virus matrix protein 2. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Influenza A virus (IAV) |
| Immunogen | Influenza a virus matrix protein 2 |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA; FuncS |
| Application Notes | The antibody is recommended for detection of IAV M2 protein by WB, ELISA, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | IAV M2 protein |
| Alternative Name | M2 protein; matrix protein 2; M2e |
| Research Area | Microbiology; Infectious Disease |
Figure 1 Recombinant Anti-IAV M2 protein Antibody (V3S-0522-YC7462) in Western Blot.
Western blot analysis of V3S-0522-YC7462 was performed by loading IAV M2 protein.
The secondary antibody: HRP anti-mouse IgG (H+L)
Lane 1: Reduced Antigen (0.1 μg)
Lane 2: Reduced Antigen (0.3 μg)
Lane 3: Reduced Antigen (0.6 μg)
Figure 1 Recombinant Anti-IAV M2 protein Antibody (V3S-0522-YC7462) in Western Blot.
Figure 2 Recombinant Anti-IAV M2 protein Antibody (V3S-0522-YC7462) in ELISA.
ELISA analysis of V3S-0522-YC7462 was performed by coating with IAV M2 protein.
The secondary antibody: HRP anti-mouse IgG (H+L)
Figure 2 Recombinant Anti-IAV M2 protein Antibody (V3S-0522-YC7462) in ELISA.
Figure 3 Recombinant Anti-IAV M2 protein Antibody (V3S-0522-YC7462) in Dot Blot.
Dot blot analysis of V3S-0522-YC7462 was performed by loading IAV M2 protein.
V3S-0522-YC7462 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-anti-mouse IgG (H+L)
Figure 3 Recombinant Anti-IAV M2 protein Antibody (V3S-0522-YC7462) in Dot Blot.
