| Description | This product is a human monoclonal antibody that reacts with IL13. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA; FC; IP; FuncS; IF; Neut; ICC |
| Application Notes | The antibody is recommended for detection of IL13 by ELISA, FC, IP, FuncS, IF, Neut, ICC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-IL13 Monoclonal Antibody (V3S-0622-YC4588) in SDS-PAGE
SDS-PAGE analysis of V3S-0622-YC4588 in non-reduced (lane 1, 2.5ug) and reduced (lane 2, 2.5ug) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 1 Anti-IL13 Monoclonal Antibody (V3S-0622-YC4588) in SDS-PAGE
Figure 2 Anti-IL13 Monoclonal Antibody (V3S-0622-YC4588) in SEC-HPLC
The purity of V3S-0622-YC4588 was greater than 95% as determined by SEC-HPLC.
Figure 2 Anti-IL13 Monoclonal Antibody (V3S-0622-YC4588) in SEC-HPLC
Figure 3 Anti-IL13 Monoclonal Antibody (V3S-0622-YC4588) in ELISA
ELISA analysis of V3S-0622-YC4588 was performed by coating with Recombinant Human IL3.
Figure 3 Anti-IL13 Monoclonal Antibody (V3S-0622-YC4588) in ELISA
Figure 4 Anti-IL13 Monoclonal Antibody (V3S-0622-YC4588) in WB
ELISA analysis of V3S-0622-YC4588 was performed by coating with Recombinant Human IL3.
Lane 1: Non-Reducing Antigen (1ug)
Lane 2: Reducing Antigen (1ug)
The secondary antibody: Goat Anti-Human IgG-HRP.
Figure 4 Anti-IL13 Monoclonal Antibody (V3S-0622-YC4588) in WB
