Recombinant Anti-IL17A Antibody (V3S-0522-YC6568) (CAT#: V3S-0522-YC6568)

Figure 1 Anti-IL17A Monoclonal Antibody (V3S-0522-YC6568) in SDS-PAGE

SDS-PAGE analysis of V3S-0522-YC6568 in β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 2 Anti-IL17A Monoclonal Antibody (V3S-0522-YC6568) in SEC-HPLC

The purity of V3S-0522-YC6568 was greater than 95% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Anti-IL17A Monoclonal Antibody (V3S-0522-YC6568) in WB

Western blot analysis of V3S-0522-YC6568 (1:500) was performed by loading human IL17A (aa 1-155). The HRP-Anti-Human IgG (H+L) (1: 6000) as a secondary antibody.

Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.4 μg)
Lane 3: Reducing Antigen (0.8 μg)

Figure 4 Anti-IL17A Monoclonal Antibody (V3S-0522-YC6568) in DB

Dot Blot analysis of V3S-0522-YC6568 (1:500) was performed by coating with human IL17A (aa 1-155). The HRP-Anti-Human IgG (H+L) (1: 6000) as a secondary antibody.

Figure 5 Anti-IL17A Monoclonal Antibody (V3S-0522-YC6568) in ELISA

ELISA analysis of V3S-0522-YC6568 was performed by coating human IL17A (aa 1-155). The HRP-Anti-Human IgG (H+L) (1: 4000) as a secondary antibody.