| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Interleukin 17 Receptor B. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human, Mouse |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; DB; ELISA; FC; Inhib; FuncS |
| Application Notes | The antibody is recommended for detection of IL17RB by ELISA, FC, Inhib, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) in DB
Dot Blot analysis of V3S-0522-YC2362 was performed by coating with human IL17RB protein (His tag).
V3S-0522-YC2362 incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)
Figure 1 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) in DB
Figure 2 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) in WB
Western blot analysis of V3S-0522-YC2362 was performed by loading human IL17RB protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC2362 and HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 2 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) in WB
Figure 3 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) in ELISA
ELISA analysis of V3S-0522-YC2362 was performed by coating with human IL17RB protein (His tag). Then blocked with BSA and incubated with V3S-0522-YC2362. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) in ELISA
Figure 4 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) Fab Fragment in DB
Dot Blot analysis of V3S-0522-YC2362 Fab fragment was performed by coating with human IL17RB protein (His tag).
V3S-0522-YC2362-F(E) incubation concentration: 2 μg/mL.
HRP conjugated Anti-His tag as a secondary antibody (1: 2000)
Figure 4 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) Fab Fragment in DB
Figure 5 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) Fab Fragment in WB
Western blot analysis of V3S-0522-YC2362 Fab fragment was performed by loading human IL17RB protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC2362-F(E) and HRP conjugated Anti-His tag as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 5 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) Fab Fragment in WB
Figure 6 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) Fab Fragment in ELISA
ELISA analysis of V3S-0522-YC2362 Fab fragment was performed by coating with human IL17RB protein (His tag). Then blocked with BSA and incubated with V3S-0522-YC2362-F(E). The HRP conjugated Anti-His tag as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 6 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) Fab Fragment in ELISA
