Anti-IL17RB Neutralizing Antibody (V3S-0522-YC2362) (CAT#: V3S-0522-YC2362)

Figure 1 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) in DB

Dot Blot analysis of V3S-0522-YC2362 was performed by coating with human IL17RB protein (His tag).
V3S-0522-YC2362 incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)

Figure 2 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) in WB

Western blot analysis of V3S-0522-YC2362 was performed by loading human IL17RB protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC2362 and HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 3 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) in ELISA

ELISA analysis of V3S-0522-YC2362 was performed by coating with human IL17RB protein (His tag). Then blocked with BSA and incubated with V3S-0522-YC2362. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 4 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) Fab Fragment in DB

Dot Blot analysis of V3S-0522-YC2362 Fab fragment was performed by coating with human IL17RB protein (His tag).
V3S-0522-YC2362-F(E) incubation concentration: 2 μg/mL.
HRP conjugated Anti-His tag as a secondary antibody (1: 2000)

Figure 5 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) Fab Fragment in WB

Western blot analysis of V3S-0522-YC2362 Fab fragment was performed by loading human IL17RB protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC2362-F(E) and HRP conjugated Anti-His tag as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 6 Mouse Anti-IL17RB Monoclonal Antibody (V3S-0522-YC2362) Fab Fragment in ELISA

ELISA analysis of V3S-0522-YC2362 Fab fragment was performed by coating with human IL17RB protein (His tag). Then blocked with BSA and incubated with V3S-0522-YC2362-F(E). The HRP conjugated Anti-His tag as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.