| Description | This product is a monoclonal antibody derived from Rat (Rattus norvegicus), which can specifically recognize Interleukin 18 (interferon-gamma-inducing factor). The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Rat |
| Target Species | Human |
| Immunogen | Recombinant IL-18 |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; DB; Neut |
| Application Notes | The antibody is recommended for detection of IL18 by Neut assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) in DB
Dot Blot analysis of V3S-0522-YC2365 was performed by coating with human IL18 protein (His tag).
V3S-0522-YC2365 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Rat IgG as a secondary antibody (1: 2000)
Figure 1 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) in DB
Figure 2 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) in WB
Western blot analysis of V3S-0522-YC2365 was performed by loading human IL18 protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC2365 and HRP goat anti-Rat IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Figure 2 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) in WB
Figure 3 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) in ELISA
ELISA analysis of V3S-0522-YC2365 was performed by coating with human IL18 protein (His tag). Then blocked with BSA and incubated with V3S-0522-YC2365. The HRP-conjugated goat anti-Rat IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) in ELISA
Figure 4 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) Fab Fragment in DB
Dot Blot analysis of V3S-0522-YC2365 Fab fragment was performed by coating with human IL18 protein (His tag).
V3S-0522-YC2365-F(E) incubation concentration: 2 μg/mL.
HRP conjugated Anti-Rat IgG (H+L) as a secondary antibody (1: 3000)
Figure 4 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) Fab Fragment in DB
Figure 5 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) Fab Fragment in WB
Western blot analysis of V3S-0522-YC2365 Fab fragment was performed by loading human IL18 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC2365-F(E) and HRP conjugated Anti-Rat IgG (H+L) as a secondary antibody (1: 3000). Chemiluminescent detection was performed.
Lane1: Reduced antigen (0.1 μg)
Lane2: Reduced antigen (0.3 μg)
Lane3: Reduced antigen (0.6 μg)
Figure 5 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) Fab Fragment in WB
Figure 6 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) Fab Fragment in ELISA
ELISA analysis of V3S-0522-YC2365 Fab fragment was performed by coating with human IL18 protein (His tag). Then blocked with BSA and incubated with V3S-0522-YC2365-F(E). The HRP conjugated Anti-His tag as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 6 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) Fab Fragment in ELISA
Figure 7 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) scFv Fragment in DB
Dot Blot analysis of V3S-0522-YC2365 scFv fragment was performed by coating with human IL18 protein (His tag).
V3S-0522-YC2365-S(P) incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-His tag as a secondary antibody (1: 2000)
Figure 7 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) scFv Fragment in DB
Figure 8 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) scFv Fragment in WB
Western blot analysis of V3S-0522-YC2365 scFv fragment was performed by loading human IL18 protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC2365-S(P) and HRP goat anti-His tag as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Figure 8 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) scFv Fragment in WB
Figure 9 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) scFv Fragment in ELISA
ELISA analysis of V3S-0522-YC2365 scFv fragment was performed by coating with human IL18 protein (His tag). Then blocked with BSA and incubated with V3S-0522-YC2365-S(P). The HRP-conjugated goat anti-His tag as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 9 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) scFv Fragment in ELISA
