Anti-IL18 Neutralizing Antibody (V3S-0522-YC2365) (CAT#: V3S-0522-YC2365)

Figure 1 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) in DB

Dot Blot analysis of V3S-0522-YC2365 was performed by coating with human IL18 protein (His tag).
V3S-0522-YC2365 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Rat IgG as a secondary antibody (1: 2000)

Figure 2 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) in WB

Western blot analysis of V3S-0522-YC2365 was performed by loading human IL18 protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC2365 and HRP goat anti-Rat IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Figure 3 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) in ELISA

ELISA analysis of V3S-0522-YC2365 was performed by coating with human IL18 protein (His tag). Then blocked with BSA and incubated with V3S-0522-YC2365. The HRP-conjugated goat anti-Rat IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 4 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) Fab Fragment in DB

Dot Blot analysis of V3S-0522-YC2365 Fab fragment was performed by coating with human IL18 protein (His tag).
V3S-0522-YC2365-F(E) incubation concentration: 2 μg/mL.
HRP conjugated Anti-Rat IgG (H+L) as a secondary antibody (1: 3000)

Figure 5 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) Fab Fragment in WB

Western blot analysis of V3S-0522-YC2365 Fab fragment was performed by loading human IL18 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC2365-F(E) and HRP conjugated Anti-Rat IgG (H+L) as a secondary antibody (1: 3000). Chemiluminescent detection was performed.

Lane1: Reduced antigen (0.1 μg)
Lane2: Reduced antigen (0.3 μg)
Lane3: Reduced antigen (0.6 μg)

Figure 6 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) Fab Fragment in ELISA

ELISA analysis of V3S-0522-YC2365 Fab fragment was performed by coating with human IL18 protein (His tag). Then blocked with BSA and incubated with V3S-0522-YC2365-F(E). The HRP conjugated Anti-His tag as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 7 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) scFv Fragment in DB

Dot Blot analysis of V3S-0522-YC2365 scFv fragment was performed by coating with human IL18 protein (His tag).
V3S-0522-YC2365-S(P) incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-His tag as a secondary antibody (1: 2000)

Figure 8 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) scFv Fragment in WB

Western blot analysis of V3S-0522-YC2365 scFv fragment was performed by loading human IL18 protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC2365-S(P) and HRP goat anti-His tag as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Figure 9 Rat Anti-IL18 Monoclonal Antibody (V3S-0522-YC2365) scFv Fragment in ELISA

ELISA analysis of V3S-0522-YC2365 scFv fragment was performed by coating with human IL18 protein (His tag). Then blocked with BSA and incubated with V3S-0522-YC2365-S(P). The HRP-conjugated goat anti-His tag as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.