| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Interleukin 4 receptor. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Cynomolgus |
| Affinity | KD = 4.5 nM, determined by surface plasmon resonance. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; DB; HTRF; FuncS |
| Application Notes | The antibody is recommended for detection of IL4R by HTRF, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-IL4R Human Monoclonal Antibody (V3S-0522-YC2458) in ELISA
ELISA analysis of V3S-0522-YC2458 was performed by coating with human IL4R protein (His tag). Then blocked with BSA and incubated with V3S-0522-YC2458. The HRP-conjugated goat anti-IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Anti-IL4R Human Monoclonal Antibody (V3S-0522-YC2458) in ELISA
Figure 2 Anti-IL4R Human Monoclonal Antibody (V3S-0522-YC2458) in DB
Dot Blot analysis of V3S-0522-YC2458 was performed by coating with human IL4R protein (His tag).
V3S-0522-YC2458 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) as a secondary antibody (1: 3000)
NC: Negative control, PC: Positive control
Figure 2 Anti-IL4R Human Monoclonal Antibody (V3S-0522-YC2458) in DB
