| Description | This antibody exhibits a high affinity for CD127, with a dissociation constant of 556 pM. Additionally, it demonstrated the ability to partially obstruct the binding of IL-7 to CD127, resulting in a corresponding partial inhibition of IL-7-induced STAT-5 phosphorylation in human CD4 cells. Furthermore, the antibody demonstrated the capability to recognize CD127 expressed on the surface of a CD127-transfected CHO cell line. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | The amino acid 21-262 of human CD127. |
| Epitope | Amino acids 21-262 |
| Affinity | KD = 556 pM |
| Isotype | Mouse IgG1, kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Neut |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
