| Sub Cat | Isotype | Application | |
|---|---|---|---|
| V3S-1124-YC1385 | IgG4 | Neut; FuncS | |
| V3S-0622-YC1958 | IgG | FuncS; IF; Neut; ELISA; FC; IP; WB | |
| V3S-0622-YC1959 | IgG | IF; IP; Neut; FuncS; ELISA; FC; ICC | |
| V3S-0622-YC303 | IgG1 | ELISA; FC; Block | |
| V3S-0522-YC7593 | IgG1 | FC; ELISA; FuncS | |
| V3S-0622-YC4841 | IgG1 kappa | ELISA |
| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Integrin alpha 4. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Human integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) |
| Isotype | IgG4 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | Neut; FuncS |
| Application Notes | The antibody is recommended for detection of ITGA4 by Neut, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | ITGA4 |
| Alternative Name | ITGA4; integrin, alpha 4 (antigen CD49D; alpha 4 subunit of VLA-4 receptor); IA4; CD49D; integrin alpha-4; 269C wild type; integrin alpha 4; integrin alpha-IV; VLA-4 subunit alpha; integrin alpha-4 subunit; CD49 antigen-like family member D; antigen CD49D; alpha-4 subunit of VLA-4 receptor; very late activation protein 4 receptor; alpha 4 subunit |
| Gene ID | 3676 |
| UniProt | P13612 |
| Research Area | Immunology; Signal Pathway |
