| Description | CNTO95 is a human IgG1, kappa monoclonal antibody that inhibits the migration of human melanoma and endothelial cells. CNTO95 has been demonstrated to bind to all subunits of the integrins that contain alpha V, except those containing a5 or aIIb heterodimers. It blocks the binding of both αVβ3 and αVβ5 to vitronectin in cell-based assays. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Affinity | KD = 0.21±0.133 nM (αVβ3), KD = 25±10.4 pM (αVβ5). |
| Isotype | Human IgG1, kappa |
| Expression Species | HEK293F or CHO |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Block |
| Application Notes | ELISA: 0.7 μg/mL |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
