| Description | This product is a human monoclonal antibody that reacts with KIT. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; DB; FuncS |
| Application Notes | The antibody is recommended for detection of KIT by FuncS assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | KIT |
| Alternative Name | KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT; SCFR; C-Kit; CD117; mast/stem cell growth factor receptor Kit; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-onco |
| Gene ID | 3815 |
| UniProt | A0A024RDA0 |
| Research Area | Cancer Research |
Figure 1 Anti-Human KIT Monoclonal Antibody (V3S-0622-YC4871) in DB
Dot Blot analysis of V3S-0622-YC4871 was performed by coating with c-Kit Protein, Human, Recombinant (His Tag).
The secondary antibody: HRP Goat Anti-Human IgG (H+L)
Figure 1 Anti-Human KIT Monoclonal Antibody (V3S-0622-YC4871) in DB
Figure 2 Anti-Human KIT Monoclonal Antibody (V3S-0622-YC4871) in WB
Western blot analysis of V3S-0622-YC4871 was performed by loading c-Kit Protein, Human, Recombinant (His Tag).
The secondary antibody: HRP Goat Anti-Human IgG (H+L)
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
Figure 2 Anti-Human KIT Monoclonal Antibody (V3S-0622-YC4871) in WB
Figure 3 Anti-Human KIT Monoclonal Antibody (V3S-0622-YC4871) in ELISA
ELISA analysis of V3S-0622-YC4871 was performed by coating with c-Kit Protein, Human, Recombinant (His Tag).
The secondary antibody: HRP Goat Anti-Human IgG (H+L)
Figure 3 Anti-Human KIT Monoclonal Antibody (V3S-0622-YC4871) in ELISA
Figure 4 Anti-KIT Monoclonal Antibody (V3S-0622-YC4871, Lot#CB2303LX10) in SDS-PAGE.
SDS-PAGE analysis of V3S-0622-YC4871 in β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 4 Anti-KIT Monoclonal Antibody (V3S-0622-YC4871, Lot#CB2303LX10) in SDS-PAGE.
Figure 5 Anti-KIT Monoclonal Antibody (V3S-0622-YC4871, Lot#CB2303LX10) in SEC-HPLC.
The purity of V3S-0622-YC4871 was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 5 Anti-KIT Monoclonal Antibody (V3S-0622-YC4871, Lot#CB2303LX10) in SEC-HPLC.
Figure 6 Anti-KIT Monoclonal Antibody (V3S-0622-YC4871, Lot#CB2303LX10) in WB.
Western blot analysis was performed by loading 0.1 µg (lane 1), 0.3 µg(lane 2), and 0.6 µg (lane 3) recombinant human KIT onto a 12% Tris-HCl polyacrylamide gel.
Figure 6 Anti-KIT Monoclonal Antibody (V3S-0622-YC4871, Lot#CB2303LX10) in WB.
Figure 7 Anti-KIT Monoclonal Antibody (V3S-0622-YC4871, Lot#CB2303LX10) in DB.
Dot Blot analysis of V3S-0622-YC4871 was performed by coating with human KIT antigen.
Figure 7 Anti-KIT Monoclonal Antibody (V3S-0622-YC4871, Lot#CB2303LX10) in DB.
Figure 8 Anti-KIT Monoclonal Antibody (V3S-0622-YC4871, Lot#CB2303LX10) in ELISA.
ELISA analysis of V3S-0622-YC4871 was performed by coating with c-Kit Protein, Human, Recombinant (ECD, His Tag).
Figure 8 Anti-KIT Monoclonal Antibody (V3S-0622-YC4871, Lot#CB2303LX10) in ELISA.
