Anti-KIT Neutralizing Antibody (V3S-0622-YC4871) (CAT#: V3S-0622-YC4871)

Figure 1 Anti-Human KIT Monoclonal Antibody (V3S-0622-YC4871) in DB

Dot Blot analysis of V3S-0622-YC4871 was performed by coating with c-Kit Protein, Human, Recombinant (His Tag).
The secondary antibody: HRP Goat Anti-Human IgG (H+L)

Figure 2 Anti-Human KIT Monoclonal Antibody (V3S-0622-YC4871) in WB

Western blot analysis of V3S-0622-YC4871 was performed by loading c-Kit Protein, Human, Recombinant (His Tag).
The secondary antibody: HRP Goat Anti-Human IgG (H+L)

Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)

Figure 3 Anti-Human KIT Monoclonal Antibody (V3S-0622-YC4871) in ELISA

ELISA analysis of V3S-0622-YC4871 was performed by coating with c-Kit Protein, Human, Recombinant (His Tag).
The secondary antibody: HRP Goat Anti-Human IgG (H+L)

Figure 4 Anti-KIT Monoclonal Antibody (V3S-0622-YC4871, Lot#CB2303LX10) in SDS-PAGE.

SDS-PAGE analysis of V3S-0622-YC4871 in β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 5 Anti-KIT Monoclonal Antibody (V3S-0622-YC4871, Lot#CB2303LX10) in SEC-HPLC.

The purity of V3S-0622-YC4871 was greater than 95% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 6 Anti-KIT Monoclonal Antibody (V3S-0622-YC4871, Lot#CB2303LX10) in WB.

Western blot analysis was performed by loading 0.1 µg (lane 1), 0.3 µg(lane 2), and 0.6 µg (lane 3) recombinant human KIT onto a 12% Tris-HCl polyacrylamide gel.

Figure 7 Anti-KIT Monoclonal Antibody (V3S-0622-YC4871, Lot#CB2303LX10) in DB.

Dot Blot analysis of V3S-0622-YC4871 was performed by coating with human KIT antigen.

Figure 8 Anti-KIT Monoclonal Antibody (V3S-0622-YC4871, Lot#CB2303LX10) in ELISA.

ELISA analysis of V3S-0622-YC4871 was performed by coating with c-Kit Protein, Human, Recombinant (ECD, His Tag).