| Description | This product is a human monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing human lysosomal associated membrane protein 1 (LAMP1). It can be used for LAMP1 detection in Enzyme-Linked Immunosorbent Assay and dot blot. The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG1 kappa |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A or G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; DB |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-Human LAMP1 Monoclonal Antibody (V3S-0822-YC3130) in ELISA
ELISA analysis of V3S-0822-YC3130 was performed by coating with Recombinant Human LAMP1 Protein (his-tag). Then blocked with BSA, and incubated with Anti-Human LAMP1 Antibody (V3S-0822-YC3130). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Anti-Human LAMP1 Monoclonal Antibody (V3S-0822-YC3130) in ELISA
Figure 2 Anti-Human LAMP1 Monoclonal Antibody (V3S-0822-YC3130) in DB
Dot blot analysis of V3S-0822-YC3130 was performed by loading Recombinant Human LAMP1 Protein (his-tag) onto a 12% Tris-HCl polyacrylamide gel.
Figure 2 Anti-Human LAMP1 Monoclonal Antibody (V3S-0822-YC3130) in DB
Figure 3 Anti-Human LAMP1 Monoclonal Antibody (V3S-0822-YC3130) in SDS-PAGE
SDS-PAGE analysis of V3S-0822-YC3130 in reduced (lane 2) and non-reduced (lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 3 Anti-Human LAMP1 Monoclonal Antibody (V3S-0822-YC3130) in SDS-PAGE
Figure 4 Anti-Human LAMP1 Monoclonal Antibody (V3S-0822-YC3130) in SEC-HPLC
The purity of V3S-0822-YC3130 was greater than 95% as determined by HPLC.
Figure 4 Anti-Human LAMP1 Monoclonal Antibody (V3S-0822-YC3130) in SEC-HPLC
