| Description | This product is a recombinant Mouse antibody provided by CreativeBiolabs. The antibody is specific for MAvian reovirus GP. It can be used for MARV GP detection in Western Blot (WB), Immunoprecipitation (IP). It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Marburg virus (MARV) |
| Epitope | Located in the sequence: DAVTE. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | WB; IP |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-MARV GP Recombinant Antibody (V3S-1022-YC4089) in Western Blot.
Western blot analysis of V3S-1022-YC4089 was performed by loading recombinant lake victoria marburgvirus envelope glycoprotein (GP).
V3S-1022-YC4089 incubation concentration: 2ng/μL.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 1 Anti-MARV GP Recombinant Antibody (V3S-1022-YC4089) in Western Blot.
Figure 2 Anti-MARV GP Recombinant Antibody (V3S-1022-YC4089) in ELISA.
ELISA analysis of V3S-1022-YC4089 was performed by coating with recombinant lake victoria marburgvirus envelope glycoprotein (GP).
Figure 2 Anti-MARV GP Recombinant Antibody (V3S-1022-YC4089) in ELISA.
Figure 3 Anti-MARV GP Recombinant Antibody (V3S-1022-YC4089) in Dot Blot.
Dot blot analysis of V3S-1022-YC4089 was performed by coating with recombinant lake victoria marburgvirus envelope glycoprotein (GP).
V3S-1022-YC4089 incubation concentration: 2 ng/μL.
Figure 3 Anti-MARV GP Recombinant Antibody (V3S-1022-YC4089) in Dot Blot.
