Recombinant Anti-MS4A1 Antibody (V3S-0522-YC7638) (CAT#: V3S-0522-YC7638)

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  • fig1
    Figure 1 Recombinant Anti-MS4A1 Antibody (V3S-0522-YC7638) scFv Fragment in FC.

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize MS4A1 molecule. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Immunogen Human membrane-spanning 4-domains, subfamily A, member 1
Isotype IgG1, κ

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application WB; ELISA; FuncS
Application Notes The antibody is recommended for detection of MS4A1 by WB (1/1000-1/10000), ELISA (1/1000-1/20000), FuncS assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target MS4A1
Alternative Name MS4A1; membrane-spanning 4-domains, subfamily A, member 1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16; B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1;
Gene ID 931
UniProt A0A024R507
Research Area Immunology; Stem Cell

Tested Data

FC

Figure 1 Recombinant Anti-MS4A1 Antibody (V3S-0522-YC7638) scFv Fragment in FC.

Flow cytometry analysis of V3S-0522-YC7638 scFv was performed by targeting the CD20+ Raji cell line. The Raji cells were incubated with the secondary antibody(5 µl/test) alone (Yellow group) or incubated with the scFv (Ab01, 50 µg/ml) for 1 h at room temperature and then with the secondary antibody (5 µl/test) for 30 min (Blue group). This result was detected and analyzed by instrument Panacyte A8.
The secondary antibody: APC anti-His Tag Antibody

Figure 1 Recombinant Anti-MS4A1 Antibody (V3S-0522-YC7638) scFv Fragment in FC.

For research use only, not directly for clinical use.
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