| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Membrane-spanning 4-domains, subfamily A, member 1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG1, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | FC; WB; ELISA; IF; FuncS; Apop; Neut |
| Application Notes | The antibody is recommended for detection of MS4A1 by WB, ELISA, IF, FuncS, Apop, Neut assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | MS4A1 |
| Alternative Name | MS4A1; membrane-spanning 4-domains, subfamily A, member 1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16; B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1; |
| Gene ID | 931 |
| UniProt | P11836 |
| Research Area | Immunology; Stem Cell |
Figure 1 Anti-MS4A1 Monoclonal Antibody (V3S-0522-YC8082) in FACS
Flow cytometry analysis of Daudi cells (0.5x106) with purified antibody V3S-0522-YC8082 (5 μl). Cells were blocked with Human TruStain FcXTM and washed with 0.5% BSA-PBS. A FITC Goat anti-human IgG (5 μl) as a secondary antibody.
Figure 1 Anti-MS4A1 Monoclonal Antibody (V3S-0522-YC8082) in FACS
