Anti-MS4A1 Neutralizing Antibody (V3S-0622-YC2218) (CAT#: V3S-0622-YC2218)

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  • fig1
    Figure 1 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC2218) in FACS

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a human monoclonal antibody that reacts with MS4A1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Immunogen Hmuan CD20 transfected NS/O cells.
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application FC; IP; ELISA; Neut; FuncS; IF; IHC
Application Notes The antibody is recommended for detection of MS4A1 by FC, IP, ELISA, Neut, FuncS, IF, IHC assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target MS4A1
Alternative Name membrane-spanning 4-domains, subfamily A, member 1; CD20; B-lymphocyte antigen CD20; B1; Bp35; MS4A2; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen
Gene ID 931
UniProt P11836
Research Area Immunology

Tested Data

FACS

Figure 1 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC2218) in FACS

Flow cytometry analysis of Daudi cells (0.5x106) with purified antibody V3S-0622-YC2218 (5 μl). Cells were blocked with Human TruStain FcXTM and washed with 0.5% BSA-PBS. A FITC Goat anti-human IgG (5 μl) as a secondary antibody.

Figure 1 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC2218) in FACS

For research use only, not directly for clinical use.
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