| Description | This product is a human monoclonal antibody that reacts with MS4A1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; IP; FC; FuncS; Neut; IF; ICC |
| Application Notes | The antibody is recommended for detection of MS4A1 by ELISA, IP, FC, FuncS, Neut, IF, ICC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4991) in ELISA
ELISA analysis of V3S-0622-YC4991 was performed by coating with Human MS4A1 Protein (His Tag). Then blocked with BSA and incubated with V3S-0622-YC4991. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4991) in ELISA
Figure 2 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4991) in WB
Western blot analysis of V3S-0622-YC4991 was performed by loading 2 µg onto a 12% Tris-HCl polyacrylamide gel in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with Goat Anti-Human IgG HRP secondary antibody at a dilution of 1: 8,000 for 75 minutes. Chemiluminescent detection was performed.
Figure 2 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4991) in WB
Figure 3 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4991) in SDS-PAGE
SDS-PAGE analysis of V3S-0622-YC4991 in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.
Figure 3 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4991) in SDS-PAGE
Figure 4 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4991) in FACS
Flow cytometry analysis of Daudi cells (0.5x106) with purified antibody V3S-0622-YC4991 (5 μl). Cells were blocked with Human TruStain FcXTM and washed with 0.5% BSA-PBS. A FITC Goat anti-human IgG (5 μl) as a secondary antibody.
Figure 4 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4991) in FACS
