| Description | This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for Mesothelin. It can be used for Mesothelin detection in Western Blot, Enzyme-Linked Immunosorbent Assay and Flow Cytometry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Mouse |
| Immunogen | Amino acids 296-600 of human mesothelin |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | WB; ELISA; FC |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-MSLN Antibody (V3S-1022-YC1811) in Western Blot.
Western blot analysis of V3S-1022-YC1811 was performed by loading Mesothelin protein, human, recombinant (His Tag).
CBL-rAb-1835FY incubation concentration: 2 ng/μL.
The secondary antibody: HRP-anti-human IgG (H+L)
Lane: Reduced antigen (0.6 μg)
Figure 1 Anti-MSLN Antibody (V3S-1022-YC1811) in Western Blot.
