| Sub Cat | Epitope | Isotype | Application | Affinity | |
|---|---|---|---|---|---|
| V3S-1124-YC237 | The epitope contains residues D287, T290, E293. | IgG2b | Neut; ELISA | 7.1 nM | |
| V3S-1022-YC4149 | The epitope contains residues E347, D349. | IgG2a | Neut; ELISA | ||
| V3S-1022-YC4148 | The epitope contains residue K284. | IgG2a | Neut; ELISA | 16.7 nM | |
| V3S-1022-YC4150 | The epitope contains residue N481. | IgG1 | Neut; ELISA | ||
| V3S-1022-YC4153 | The epitope contains residue P325. | IgG2a | Neut; ELISA | ||
| V3S-1022-YC4151 | The epitope contains residues P454, Q456, A457. | IgG1 | Neut; ELISA | ||
| V3S-1022-YC4152 | The epitope contains residue R460. | IgG1 | Neut; ELISA |
| Description | This product is a recombinant Mouse antibody provided by CreativeBiolabs. The antibody is specific for Newcastle disease virus strain Beaudette C/45 Hemagglutinin-neuraminidase. It can be used for NDV HN detection in Enzyme-linked Immunosorbent Assay (ELISA), Neutralization (Neut). It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | NDV strain Beaudette C/45 |
| Epitope | The epitope contains residues D287, T290, E293. |
| Affinity | The affinity is in nanomolar range, about 7.1 nM, determined by surface plasmon resonance. |
| Isotype | IgG2b |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; Neut |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | NDV HN |
| Alternative Name | HN; Hemagglutinin-neuraminidase; NDV strain Beaudette C/45 HN; Newcastle disease virus strain Beaudette C/45 |
| UniProt | P32884 |
| Research Area | Microbiology |
