Recombinant Anti-NRP1 Antibody (V3S-1022-YC1540) (CAT#: V3S-1022-YC1540)

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Datasheet

MSDS

COA

Sub Cat Immunogen Epitope Isotype Application Affinity  
V3S-1124-YC439 Full length human IgG1 IgG FC; ELISA Inquiry
V3S-1022-YC3189 CHO cell expressed hNRP1-Fc protein In the a1a2 domain of hNRP1. IgG1 ELISA; FC; Block 0.9 nM Inquiry
V3S-0522-YC6710 IgG1 FC; Block 0.4 nM Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a recombinant human antibody provided by CreativeBiolabs. The antibody is specific for Neuropilin 1, this gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. It can be used for NRP1 detection in Flow Cytometry, Enzyme-Linked Immunosorbent Assay. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Human
Target Species Human, Mouse
Immunogen Full length human IgG1
Isotype IgG

Property

Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application FC; ELISA

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target NRP1
Alternative Name Neuropilin 1; Vascular Endothelial Cell Growth Factor 165 Receptor; VEGF165R; NRP; Transmembrane Receptor; CD304 Antigen
Gene ID 8829
UniProt O14786
Research Area Cell Biology
For research use only, not directly for clinical use.
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