| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Osteopontin. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Human Osteopontin |
| Isotype | IgG, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA; FuncS |
| Application Notes | The antibody is recommended for detection of OPN by WB, ELISA, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | OPN |
| Alternative Name | OPN; Osteopontin; bone sialoprotein I; BSP-1; BNSP; early T-lymphocyte activation; ETA-1; secreted phosphoprotein 1; SPP1; Ric |
| Research Area | Cell Biology |
Figure 1 Mouse Anti-OPN Recombinant Antibody (V3S-0522-YC7525) in WB
Western blot analysis of V3S-0522-YC7525 was performed by loading recombinant human OPN protein.
The incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-mouse IgG
Lane 1: Reduced Antigen (0.1 μg)
Lane 2: Reduced Antigen (0.3 μg)
Lane 3: Reduced Antigen (0.6 μg)
Figure 1 Mouse Anti-OPN Recombinant Antibody (V3S-0522-YC7525) in WB
