| Description | This product is a fully human antibody that recognizes human PDCD1. The purified mAb binds to human and cynomogous PD-1 proteins with affinities of 36 pM and 45 pM by ELISA, respectively. The mAb effectively blocks the interaction between PD-1 and PD-L1 with an IC₅₀ of 1.6 nM. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Monkey |
| IC50 | 1.6 nM |
| Affinity | KD = 36 pM (human) KD = 45 pM (cynomogous) |
| Isotype | Human IgG4, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Block; SPR |
| Application Notes | Block: this antibody blocks the interaction of PD-1 and PD-L1 with an IC₅₀ of 1.6nM. SPR: the capture of PD-1 was followed by two-fold dilution series of mAb059c from 200 nM to 3.125 nM (50 nM to 1.5 nM for PD1-hFc). |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | PDCD1 |
| Alternative Name | PDCD1; HPD-L; CD279; PD1; HSLE1; CD279 Antigen; HPD-1; PD-1; programmed cell death 1; programmed cell death protein 1; programmed cell death 1 protein; protein PD-1; systemic lupus erythematosus susceptibility 2 |
| Gene ID | 5133 |
| UniProt | Q15116 |
| Research Area | Cell Biology |
