| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Plasminogen Activator, Urokinase Receptor. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Human plasminogen activator, urokinase receptor |
| Isotype | IgG1, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA; FuncS |
| Application Notes | The antibody is recommended for detection of PLAUR by WB, ELISA, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | PLAUR |
| Alternative Name | PLAUR; plasminogen activator; urokinase receptor; CD87; UPAR; URKR; U-PAR; urokinase plasminogen activator surface receptor; monocyte activation antigen Mo3; u-plasminogen activator receptor form 2; urokinase-type plasminogen activator (uPA) receptor |
| Gene ID | 5329 |
| UniProt | Q03405 |
| Research Area | Cardiovascular; Immunology |
Figure 1 Anti-PLAUR Monoclonal Antibody (V3S-0522-YC7588) in SDS-PAGE
SDS-PAGE analysis of V3S-0522-YC7588 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180 kDa.
Figure 1 Anti-PLAUR Monoclonal Antibody (V3S-0522-YC7588) in SDS-PAGE
Figure 2 Anti-PLAUR Monoclonal Antibody (V3S-0522-YC7588) in SEC-HPLC
The purity of V3S-0522-YC7588 was greater than 86.8% as determined by SEC-HPLC.
Figure 2 Anti-PLAUR Monoclonal Antibody (V3S-0522-YC7588) in SEC-HPLC
Figure 3 Anti-PLAUR Monoclonal Antibody (V3S-0522-YC7588) in WB
Western blot analysis of V3S-0522-YC7588 was performed by loading Recombinant Human PLAUR Protein (lane 2, 1 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC7588 and HRP Goat Anti-Mouse IgG as a secondary antibody. Chemiluminescent detection was performed.
Figure 3 Anti-PLAUR Monoclonal Antibody (V3S-0522-YC7588) in WB
Figure 4 Anti-PLAUR Monoclonal Antibody (V3S-0522-YC7588) in ELISA
ELISA analysis of V3S-0522-YC7588 was performed by coating with Recombinant Human PLAUR Protein. Then blocked with BSA, and incubated with Anti-Human PLAUR Antibody (V3S-0522-YC7588). The HRP-conjugated goat anti-Mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Anti-PLAUR Monoclonal Antibody (V3S-0522-YC7588) in ELISA
