Recombinant Anti-PMEL Antibody (V3S-0522-YC6300) (CAT#: V3S-0522-YC6300)

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Datasheet

MSDS

COA

Sub Cat Target Species Immunogen Isotype Application  
V3S-1124-YC201 Human, Cynomolgus, Mouse, Rat IgG ELISA; WB; FC Inquiry
V3S-0622-YC2435 Human Recombinant PMEL-GST fusion protein IgG2b IHC; WB; FC Inquiry
V3S-0622-YC2436 Human Recombinant PMEL-GST fusion protein IgG1 IHC; WB; FC Inquiry
V3S-0622-YC2437 Human Recombinant PMEL-GST fusion protein IgG2a IHC; WB; FC Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Premelanosome protein. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human, Cynomolgus, Mouse, Rat
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application ELISA; WB; FC
Application Notes The antibody is recommended for detection of PMEL by ELISA, WB, FC assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target PMEL
Alternative Name P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
Gene ID 6490
UniProt P40967
Research Area Signal Pathway
Related Disease Melanoma
For research use only, not directly for clinical use.
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