Recombinant Anti-Prnp (aa 156-165 Antibody (V3S-0522-YC4620) (CAT#: V3S-0522-YC4620)

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Datasheet

MSDS

COA

Sub Cat Epitope Isotype Application  
V3S-1124-YC920 The epitope contains residues Y156, E157, D158, R159, Y160, M165. IgG1 WB Inquiry
V3S-0522-YC4619 The epitope contains residues Y156, E157, D158, R159, Y160, M165. IgG2a IF Inquiry
V3S-0522-YC4621 The epitope contains residues Y156, E157, D158, R159, Y160, M165. IgG WB Inquiry
V3S-0522-YC4378 Located in the sequence: GSDYEDRYYRENM. IgG1 ELISA Inquiry
V3S-0522-YC4597 Located in the sequence: YEDRY. IgG1 IHC Inquiry
V3S-0522-YC4958 Located in the sequence: GSDYEDRYYRENM. IgG2b ELISA Inquiry
V3S-1022-YC3376 Located in the sequence: THGQWNKPSK. IgG1 ELISA Inquiry
V3S-1022-YC3447 Located in the sequence: QVYYRPVDQ. IgG WB; ELISA; IHC Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Major prion protein precursor. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Cattle
Epitope The epitope contains residues Y156, E157, D158, R159, Y160, M165.
Isotype IgG1

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application WB
Application Notes The antibody is recommended for detection of Prnp by WB assay.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target Prnp
Alternative Name Prnp; Major prion protein precursor; Cattle; Cattle; Major prion protein precursor; Prnp
Gene ID 281427
UniProt P10279
Research Area Microbiology; Neuroscience
Related Disease Transmissible spongiform encephalopathies
For research use only, not directly for clinical use.
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