This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Prion protein. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality
Monoclonal
Host Species
Mouse
Target Species
Mouse
Immunogen
Mouse prion protein
Epitope
An epitope containing the sequence GQPHGGG/SW.
Isotype
IgG
Property
Expression Species
HEK293F or CHO
Conjugation
None
Purity
>95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin
<1 EU/mg, determined by LAL method
Purification
Protein A affinity purified
Sterility
0.2 μM filtered
Formulation
PBS, pH 7.4
Preservation
No preservatives
Stabilizer
No stabilizers
Storage
Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.
Applications
Application
WB; IHC; Inhib
Application Notes
The antibody is recommended for detection of PRNP by WB, IHC, Inhib assays.
