Recombinant Anti-ROR1 Antibody (V3S-1022-YC2493) (CAT#: V3S-1022-YC2493)

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Datasheet

MSDS

COA

Sub Cat Epitope Isotype Application Affinity  
V3S-1124-YC238 In the KNDAPVVQEPRRLSFRSTIYGSR and AANCIRIGIPMADPI. IgG1 kappa ELISA; FC 17 pM Inquiry
V3S-1022-YC2494 In the SSTGVLFVKFGPPPTASPG and SNPMILMRLKLPNCE. IgG1 kappa ELISA; FC 15 pM Inquiry
V3S-1022-YC2495 In the SSTGVLFVKFGPPPTASPG and SNPMILMRLKLPNCE. IgG1 kappa ELISA; FC 8.6 pM Inquiry
V3S-1022-YC2490 In the ECD domain. IgG1 ELISA; FC 0.19 nM Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for Receptor tyrosine kinase like orphan receptor 1. It can be used for Receptor tyrosine kinase like orphan receptor 1 detection in Enzyme-Linked Immunosorbent Assay and Flow Cytometry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Human
Target Species Human, Mouse
Immunogen The human ROR1 extracellular domain (ECD) protein
Epitope The epitope is in the KNDAPVVQEPRRLSFRSTIYGSR and AANCIRIGIPMADPI.
Affinity The affinity is in picomolar range, about 17 pM, determined by surface plasmon resonance.
Isotype IgG1 kappa

Property

Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application ELISA; FC

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target ROR1
Alternative Name ROR1; receptor tyrosine kinase-like orphan receptor 1; NTRKR1; dJ537F10.1; tyrosine-protein kinase transmembrane receptor ROR1; neurotrophic tyrosine kinase, receptor-related 1
Gene ID 4919
UniProt Q01973
Research Area Signal Pathway; Neuroscience
Related Disease Cancer
For research use only, not directly for clinical use.
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