Sub Cat | Epitope | Isotype | Application | Affinity | |
---|---|---|---|---|---|
V3S-1124-YC238 | In the KNDAPVVQEPRRLSFRSTIYGSR and AANCIRIGIPMADPI. | IgG1 kappa | ELISA; FC | 17 pM | |
V3S-1022-YC2494 | In the SSTGVLFVKFGPPPTASPG and SNPMILMRLKLPNCE. | IgG1 kappa | ELISA; FC | 15 pM | |
V3S-1022-YC2495 | In the SSTGVLFVKFGPPPTASPG and SNPMILMRLKLPNCE. | IgG1 kappa | ELISA; FC | 8.6 pM | |
V3S-1022-YC2490 | In the ECD domain. | IgG1 | ELISA; FC | 0.19 nM |
Description | This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for Receptor tyrosine kinase like orphan receptor 1. It can be used for Receptor tyrosine kinase like orphan receptor 1 detection in Enzyme-Linked Immunosorbent Assay and Flow Cytometry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
Clonality | Monoclonal |
Host Species | Human |
Target Species | Human, Mouse |
Immunogen | The human ROR1 extracellular domain (ECD) protein |
Epitope | The epitope is in the KNDAPVVQEPRRLSFRSTIYGSR and AANCIRIGIPMADPI. |
Affinity | The affinity is in picomolar range, about 17 pM, determined by surface plasmon resonance. |
Isotype | IgG1 kappa |
Expression Species | HEK293F or CHO Cell line |
Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
Endotoxin | <1 EU/mg, determined by LAL method |
Purification | Purified with Protein A or G affinity chromatography |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
Application | ELISA; FC |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | ROR1 |
Alternative Name | ROR1; receptor tyrosine kinase-like orphan receptor 1; NTRKR1; dJ537F10.1; tyrosine-protein kinase transmembrane receptor ROR1; neurotrophic tyrosine kinase, receptor-related 1 |
Gene ID | 4919 |
UniProt | Q01973 |
Research Area | Signal Pathway; Neuroscience |
Related Disease | Cancer |