Recombinant Anti-ROR1 (aa 24-403) Antibody (V3S-0522-YC2581) (CAT#: V3S-0522-YC2581)

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Datasheet

MSDS

COA

Sub Cat Immunogen Epitope Isotype Application Affinity  
V3S-1124-YC443 AVi-6His fusion protein with the extracellular domain of human RORI (24-403) In the ECD domain. IgG ELISA; FC; Cyt; FuncS Inquiry
V3S-1022-YC2491 The three extracellular domains of human ROR1 expressed with the Fc domain of human IgG1 (Fc-hROR1) In the ECD domain. IgG1 ELISA; FC 0.11 nM Inquiry
V3S-0522-YC2582 In the Kringle domain. IgG4 kappa ELISA; FC 1.6 nM Inquiry
V3S-0522-YC2584 IgG4 kappa ELISA; FC 0.4 nM Inquiry
V3S-0522-YC6530 IgG1 ELISA; FC Inquiry
V3S-0522-YC6531 ROR1 ECD In the extracellular domain IgG ELISA; FC 5 pM Inquiry
V3S-0522-YC6535 An epitope containing the sequence EWSSTGVLFVKFGPC. IgG ELISA; FC; IF; IHC Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Receptor Tyrosine Kinase Like Orphan Receptor 1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Immunogen AVi-6His fusion protein with the extracellular domain of human RORI (24-403)
Epitope Within the ECD domain.
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application ELISA; FC; Cyt; FuncS
Application Notes The antibody is recommended for detection of ROR1 by ELISA, FC, Cyt, FuncS assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target ROR1
Alternative Name Receptor Tyrosine Kinase Like Orphan Receptor 1; Neurotrophic Tyrosine Kinase, Receptor-Related 1; NTRKR1; Inactive Tyrosine-Protein Kinase Transmembrane Receptor ROR1; EC 2.7.10.1; DJ537F10.1;
Gene ID 4919
UniProt Q01973
Research Area Signal Pathway; Neuroscience
For research use only, not directly for clinical use.
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