Sub Cat | Immunogen | Epitope | Isotype | Application | Affinity | |
---|---|---|---|---|---|---|
V3S-1124-YC443 | AVi-6His fusion protein with the extracellular domain of human RORI (24-403) | In the ECD domain. | IgG | ELISA; FC; Cyt; FuncS | ||
V3S-1022-YC2491 | The three extracellular domains of human ROR1 expressed with the Fc domain of human IgG1 (Fc-hROR1) | In the ECD domain. | IgG1 | ELISA; FC | 0.11 nM | |
V3S-0522-YC2582 | In the Kringle domain. | IgG4 kappa | ELISA; FC | 1.6 nM | ||
V3S-0522-YC2584 | IgG4 kappa | ELISA; FC | 0.4 nM | |||
V3S-0522-YC6530 | IgG1 | ELISA; FC | ||||
V3S-0522-YC6531 | ROR1 ECD | In the extracellular domain | IgG | ELISA; FC | 5 pM | |
V3S-0522-YC6535 | An epitope containing the sequence EWSSTGVLFVKFGPC. | IgG | ELISA; FC; IF; IHC |
Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Receptor Tyrosine Kinase Like Orphan Receptor 1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
Clonality | Monoclonal |
Host Species | Human |
Target Species | Human |
Immunogen | AVi-6His fusion protein with the extracellular domain of human RORI (24-403) |
Epitope | Within the ECD domain. |
Isotype | IgG |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
Endotoxin | <1 EU/mg, determined by LAL method |
Purification | Protein A affinity purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
Application | ELISA; FC; Cyt; FuncS |
Application Notes | The antibody is recommended for detection of ROR1 by ELISA, FC, Cyt, FuncS assays. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | ROR1 |
Alternative Name | Receptor Tyrosine Kinase Like Orphan Receptor 1; Neurotrophic Tyrosine Kinase, Receptor-Related 1; NTRKR1; Inactive Tyrosine-Protein Kinase Transmembrane Receptor ROR1; EC 2.7.10.1; DJ537F10.1; |
Gene ID | 4919 |
UniProt | Q01973 |
Research Area | Signal Pathway; Neuroscience |