| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Respiratory syncytial virus. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Respiratory syncytial virus (RSV) |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | DB; WB; ELISA; Neut; FuncS |
| Application Notes | The antibody is recommended for detection of RSV by WB, ELISA, Neut, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | RSV |
| Alternative Name | RSV; Human respiratory syncytial virus |
| Research Area | Microbiology; Infectious Disease |
Figure 1 Anti-RSV Monoclonal Antibody (V3S-0522-YC7555) in WB
Western blot analysis of V3S-0522-YC7555 was performed by loading Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC7555 and HRP Goat Anti-Human IgG (H+L) as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (1 μg)
Figure 1 Anti-RSV Monoclonal Antibody (V3S-0522-YC7555) in WB
Figure 2 Anti-RSV Monoclonal Antibody (V3S-0522-YC7555) in DB
Dot Blot analysis of V3S-0522-YC7555 was performed by coating with Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag).
V3S-0522-YC7555 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG (H+L) as a secondary antibody (1: 2000)
Figure 2 Anti-RSV Monoclonal Antibody (V3S-0522-YC7555) in DB
Figure 3 Anti-RSV Monoclonal Antibody (V3S-0522-YC7555) in ELISA
ELISA analysis of V3S-0522-YC7555 was performed by coating with Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag). Then blocked with BSA, and incubated with V3S-0522-YC7555. The HRP-conjugated goat anti-human IgG (H+L) as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-RSV Monoclonal Antibody (V3S-0522-YC7555) in ELISA
Figure 4 Anti-RSV Monoclonal Antibody (V3S-0522-YC7555) in WB
Western blot analysis was performed by loading human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag) (11049-V08B) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-RSV Antibody (V3S-0522-YC7555) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
Figure 4 Anti-RSV Monoclonal Antibody (V3S-0522-YC7555) in WB
Figure 5 Anti-RSV Monoclonal Antibody (V3S-0522-YC7555) in ELISA
ELISA analysis of V3S-0522-YC7555 was performed by coating with Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag) (11049-V08B). Then blocked with BSA and incubated with Anti-RSV Antibody (V3S-0522-YC7555) at a starting concentration of 1 ng/mL. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 5 Anti-RSV Monoclonal Antibody (V3S-0522-YC7555) in ELISA
