Anti-RSV Neutralizing Antibody (V3S-0522-YC7555) (CAT#: V3S-0522-YC7555)

Figure 1 Anti-RSV Monoclonal Antibody (V3S-0522-YC7555) in WB

Western blot analysis of V3S-0522-YC7555 was performed by loading Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC7555 and HRP Goat Anti-Human IgG (H+L) as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (1 μg)

Figure 2 Anti-RSV Monoclonal Antibody (V3S-0522-YC7555) in DB

Dot Blot analysis of V3S-0522-YC7555 was performed by coating with Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag).

V3S-0522-YC7555 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG (H+L) as a secondary antibody (1: 2000)

Figure 3 Anti-RSV Monoclonal Antibody (V3S-0522-YC7555) in ELISA

ELISA analysis of V3S-0522-YC7555 was performed by coating with Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag). Then blocked with BSA, and incubated with V3S-0522-YC7555. The HRP-conjugated goat anti-human IgG (H+L) as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 4 Anti-RSV Monoclonal Antibody (V3S-0522-YC7555) in WB

Western blot analysis was performed by loading human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag) (11049-V08B) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-RSV Antibody (V3S-0522-YC7555) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)

Figure 5 Anti-RSV Monoclonal Antibody (V3S-0522-YC7555) in ELISA

ELISA analysis of V3S-0522-YC7555 was performed by coating with Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag) (11049-V08B). Then blocked with BSA and incubated with Anti-RSV Antibody (V3S-0522-YC7555) at a starting concentration of 1 ng/mL. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.