| Description | This product is a monoclonal antibody derived from mouse, which can specifically recognize S100A4. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Mouse |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; DB; FuncS |
| Application Notes | The antibody is recommended for detection of S100A4 by FuncS assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | S100A4 |
| Alternative Name | S100A4; S100 calcium binding protein A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98; protein S100-A4; protein Mts1; fibroblast-specific protein-1; placental calcium-binding protein; malignant transformation suppression 1; leukemia multidrug resistance associated protein; S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) |
| Gene ID | 6275 |
| UniProt | P26447 |
| Research Area | Cardiovascular |
Figure 1 Anti-Mouse S100A4 Monoclonal Antibody (V3S-0622-YC2540) in ELISA
ELISA analysis of V3S-0622-YC2540 was performed by coating with Recombinant Mouse S100A4 Protein. Then blocked with BSA, and incubated with Anti-Mouse S100A4 Antibody (V3S-0622-YC2540). The HRP-conjugated goat anti-mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Anti-Mouse S100A4 Monoclonal Antibody (V3S-0622-YC2540) in ELISA
Figure 2 Anti-Mouse S100A4 Monoclonal Antibody (V3S-0622-YC2540) in WB
Western blot analysis of V3S-0622-YC2540 was performed by loading Recombinant Mouse S100A4 Protein (lane 1, 0.1 μg; lane 2, 0.3 μg; lane 3, 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC2540 and HRP Goat Anti-Mouse IgG as a secondary antibody. Chemiluminescent detection was performed.
Figure 2 Anti-Mouse S100A4 Monoclonal Antibody (V3S-0622-YC2540) in WB
Figure 3 Anti-Mouse S100A4 Monoclonal Antibody (V3S-0622-YC2540) in DB
Antigen: Recombinant mouse S100A4 protein
Antibody incubation concentration: 2ng/μL.
The secondary antibody: HRP-conjugated goat anti-mouse IgG
Figure 3 Anti-Mouse S100A4 Monoclonal Antibody (V3S-0622-YC2540) in DB
