| Description | The antibody recognizes both mouse and human S100A4 protein. The antibody bound both of the recombinant peptide (aa 66-89) and native protein of S100A4. It has metastasis-blocking activity and is to block the S100A4-stimulated invasion of fibroblasts. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human, Mouse |
| Epitope | AA 66-89 of human S100A4 protein. |
| Affinity | KD = 0.1 nM |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; DB; FuncS |
| Application Notes | Western blot (1 μg/mL) Immunohistochemistry (1 μg/mL) |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | S100A4 |
| Alternative Name | S100A4; S100 calcium binding protein A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98; protein S100-A4; protein Mts1; fibroblast-specific protein-1; placental calcium-binding protein; malignant transformation suppression 1; leukemia multidrug resistance associated protein; S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) |
| Gene ID | 6275 |
| UniProt | P26447 |
| Research Area | Cardiovascular |
Figure 1 Anti-Mouse S100A4 Monoclonal Antibody (V3S-0622-YC2540) in ELISA
ELISA analysis of V3S-0622-YC2540 was performed by coating with Recombinant Mouse S100A4 Protein. Then blocked with BSA, and incubated with Anti-Mouse S100A4 Antibody (V3S-0622-YC2540). The HRP-conjugated goat anti-mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Anti-Mouse S100A4 Monoclonal Antibody (V3S-0622-YC2540) in ELISA
Figure 2 Anti-Mouse S100A4 Monoclonal Antibody (V3S-0622-YC2540) in WB
Western blot analysis of V3S-0622-YC2540 was performed by loading Recombinant Mouse S100A4 Protein (lane 1, 0.1 μg; lane 2, 0.3 μg; lane 3, 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC2540 and HRP Goat Anti-Mouse IgG as a secondary antibody. Chemiluminescent detection was performed.
Figure 2 Anti-Mouse S100A4 Monoclonal Antibody (V3S-0622-YC2540) in WB
Figure 3 Anti-Mouse S100A4 Monoclonal Antibody (V3S-0622-YC2540) in DB
Antigen: Recombinant mouse S100A4 protein
Antibody incubation concentration: 2ng/μL.
The secondary antibody: HRP-conjugated goat anti-mouse IgG
Figure 3 Anti-Mouse S100A4 Monoclonal Antibody (V3S-0622-YC2540) in DB
