| Description | The antibody PVI.V6-14 binds to SARS-CoV-2 spike protein. It binds a novel, conserved epitope on the N-terminal domain (NTD) and neutralizes the original viral strain. It also binds the newly emerged variants B.1.1.7 (alpha), B.1.351 (beta), B.1.617.2 (delta), and B.1.1.529 (omicron). |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Cross Reactivity | B.1.1.7 (alpha), B.1.351 (beta), B.1.617.2 (delta), and B.1.1.529 (omicron) |
| Epitope | N-terminal domain (NTD) |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | Twenty-five nanograms of the SARS-CoV-2 spike proteins was adhered to high-capacity-binding, 96-well plates overnight in PBS. Plates were blocked with 5% BSA in PBS containing Tween 20 for 1 h at RT. The blocking solution was discarded, 3-fold dilutions of mAb PVI.V6-14 in PBS were added to wells, and the plates were incubated for 1 h at RT. The plates were then washed three times with PBS-T. Anti-human IgG-biotin in PBS-T was added to each well, and the plates were incubated for 1 h at RT. The plates were then washed three times with PBS-T. Streptavidin-HRP in PBS-T was added to each well, and the plates were incubated for 1 h at RT. The plates were then washed three times with PBS-T. Plates were developed using the 1-step Ultra TMB substrate, stopped with sulfuric acid, and immediately read using a plate reader at 450 nm. Data were plotted in Prism 9, and the area under the curve (AUC) was calculated. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
