| Description | The antibody nCoV617 is a human mAb specific for SARS-CoV-2 Spike S1 protein. It demonstrates the most potent neutralizing activity against authentic SARS-CoV-2 infection, as well as prophylactic and therapeutic efficacities against the human ACE2 transgenic mouse model in vivo. It mainly binds to the back of the "ridge" of RBD and shares limited binding residues with ACE2. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Epitope | Binds to the back of the "ridge" of RBD |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | All protein antigens were used at 200 ng/well to coat 96-well high-binding ELISA plates using carbonate-bicarbonate buffer at pH 9.6. Plates were incubated overnight at 4°C and blocked at room temperature for 2 h with PBS blocking buffer contain_x0002_ing 5% wt/vol goat serum and 0.05% Tween 20. Plasma or supernatant of transfected 293T cell cultures or purified MAbs in serial dilutions in PBS were incubated at 37°C for 1 h. The secondary antibody, goat_x0002_anti-human IgG-HRP, diluted in blocking buffer was added and incubated at 37°C for 1 h. These plates were then washed 5 times with PBS and developed with 100 μL/well TMB substrate. The reaction of the mixtures in the plates was stopped and read at a wavelength of an optical density at 450 nm by an ELISA reader. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
