| Description | The antibody NT-193 is a human mAb specific for SARS-CoV-2 Spike S1 protein. It potently neutralize SARS-CoV-2. It inhibited RBD binding to human ACE2, suggesting their epitopes overlap receptor-binding site. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Epitope | Overlap receptor-binding site and inhibites RBD binding to human ACE2 |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | ELISA plates were coated with either RBDs of CoV1, CoV2, or MERS-CoV; or S-trimer proteins of CoV1, CoV2, MERS-CoV, HCoV_x005f_x005F_x005f_x005f_x005F_x005f_x005f_x005F_x005f_x0002_NL63, HCoV-OC43, HCoV-229E, or HCoV-HKU1 at 2 μg/mL. In some experiments, the concentration of coating antigen was changed to 0.1 μg/mL. After blocking with PBS containing 1% BSA, serially-diluted sera, culture supernatants of single cells, or monoclonal antibodies were applied to the plates, and then incubated with goat anti-human IgG-HRP. HRP-activity was visualized with an OPD substrate, and OD490 was measured using an iMark Microplate Reader. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
