| Description | The antibody R1-32 is a human mAb specific for SARS-CoV-2 Spike S1 protein. It targets a semi-cryptic epitope in the spike receptor-binding domain. Binding to this non-ACE2 competing epitope results in spike destruction, thereby inhibiting virus entry. It exhibited comparable or better neutralization activity towards wild-type SARS-CoV-2 authentic virus compared with other RBD antibodies already characterized. The antibody exhibits the strongest pseudovirus neutralizing activity (IC90 = 9.95 nM), without inhibiting spike ACE2 binding. It has protection activity towards SARS-CoV-2 infection. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Epitope | Receptor-binding domain (RBD) |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | To assess antibody binding properties, 96-well ELISA plates were coated with SARS-CoV-2 RBD at 1 µg/ ml in PBS at 4 °C overnight. After standard washing and blocking procedures, 100 µl antibodies in semilogarithmic serial dilutions were added and incubated at 37°C for 2 h. After washing plates 3 times with PBS-0.05% Tween-20, plates were incubated with 1:5,000-diluted HRP-labelled goat anti-human IgG (H+L) in PBS-5% skimmed milk at 37 °C for 1 h. Plates were washed with PBS-0.05% Tween-20 6 times, TMB solution was used as the substrate, and absorbance at 450 nm was measured in a microplate reader. The antibody exhibits the strongest pseudovirus neutralizing activity (IC90 = 9.95 nM), without inhibiting spike ACE2 binding. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
