Recombinant Anti-SIGLEC10 (ECD domain) Antibody (V3S-0622-YC3862) (CAT#: V3S-0622-YC3862)

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  • fig1
    Figure 1 Mouse Anti-SIGLEC10 Monoclonal Antibody (V3S-0622-YC3862) in ELISA
  • fig1
    Figure 2 Mouse Anti-SIGLEC10 Monoclonal Antibody (V3S-0622-YC3862) in WB
  • fig1
    Figure 3 Mouse Anti-SIGLEC10 Monoclonal Antibody (V3S-0622-YC3862) in DB

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from mouse, which can specifically recognize SIGLEC10. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Immunogen Human SIGLEC10 Fc extracellular domain recombinant protein
Epitope Within the ECD domain.
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application ELISA; WB; DB; FC
Application Notes The antibody is recommended for detection of SIGLEC10 by FC assay.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target SIGLEC10
Alternative Name Sialic Acid Binding Ig Like Lectin 10; Siglec-Like Protein 2; SLG2; Sialic Acid Binding Ig-Like Lectin 10; PRO940
Gene ID 89790
UniProt Q96LC7

Tested Data

ELISA

Figure 1 Mouse Anti-SIGLEC10 Monoclonal Antibody (V3S-0622-YC3862) in ELISA

ELISA analysis of V3S-0622-YC3862 was performed by coating with human SIGLEC10 protein (His tag). Then blocked with BSA and incubated with V3S-0622-YC3862. The HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 1 Mouse Anti-SIGLEC10 Monoclonal Antibody (V3S-0622-YC3862) in ELISA

WB

Figure 2 Mouse Anti-SIGLEC10 Monoclonal Antibody (V3S-0622-YC3862) in WB

Western blot analysis of V3S-0622-YC3862 was performed by loading human SIGLEC10 protein (Lane 1 0.1 μg, Lane 2 0.3 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC3862 and HRP goat anti-mouse IgG as a secondary antibody (1: 3000). Chemiluminescent detection was performed.

Figure 2 Mouse Anti-SIGLEC10 Monoclonal Antibody (V3S-0622-YC3862) in WB

DB

Figure 3 Mouse Anti-SIGLEC10 Monoclonal Antibody (V3S-0622-YC3862) in DB

Dot Blot analysis of V3S-0622-YC3862 was performed by coating with human SIGLEC10 protein (His tag).

V3S-0622-YC3862 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 3000)

Figure 3 Mouse Anti-SIGLEC10 Monoclonal Antibody (V3S-0622-YC3862) in DB

For research use only, not directly for clinical use.
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