Anti-SIGLEC15 (Bin 5) Neutralizing Antibody (V3S-0522-YC2743) (CAT#: V3S-0522-YC2743)

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  • fig1
    Figure 1 Anti-SIGLEC15 Monoclonal Antibody (V3S-0522-YC2743) in WB
  • fig1
    Figure 2 Anti-SIGLEC15 Monoclonal Antibody (V3S-0522-YC2743) in DB
  • fig1
    Figure 3 Anti-SIGLEC15 Monoclonal Antibody (V3S-0522-YC2743) in ELISA

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Sialic Acid Binding Ig Like Lectin 15. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human, Mouse
Immunogen Human siglec 15 extracellular domain [ECD] fused with mouse IgG2a
Epitope The epitope is in the Bin 5.
Affinity KD = 1.19 nM, determined by surface plasmon resonance.
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application ELISA; WB; DB; FC; Block
Application Notes The antibody is recommended for detection of SIGLEC15 by FC, Block assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target SIGLEC15
Alternative Name Sialic Acid Binding Ig Like Lectin 15; CD33 Antigen-Like 3; CD33 Molecule-Like 3; SIGLEC-15; CD33L3; Sialic Acid Binding Ig-Like Lectin 15; Sialic Acid-Binding Ig-Like Lectin 15; HsT1361
Gene ID 284266
UniProt Q6ZMC9
Research Area Signal Pathway; Immunology

Tested Data

WB

Figure 1 Anti-SIGLEC15 Monoclonal Antibody (V3S-0522-YC2743) in WB

Western blot analysis of V3S-0522-YC2743 was performed by loading Human Siglec-15 / CD33L3 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC2743 and HRP Goat Anti-Mouse IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (1 μg)

Figure 1 Anti-SIGLEC15 Monoclonal Antibody (V3S-0522-YC2743) in WB

DB

Figure 2 Anti-SIGLEC15 Monoclonal Antibody (V3S-0522-YC2743) in DB

Dot Blot analysis of V3S-0522-YC2743 was performed by coating with Human Siglec-15 / CD33L3 Protein (His Tag).
V3S-0522-YC2743 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)

Figure 2 Anti-SIGLEC15 Monoclonal Antibody (V3S-0522-YC2743) in DB

ELISA

Figure 3 Anti-SIGLEC15 Monoclonal Antibody (V3S-0522-YC2743) in ELISA

ELISA analysis of V3S-0522-YC2743 was performed by coating with Human Siglec-15 / CD33L3 Protein (His Tag). Then blocked with BSA, and incubated with V3S-0522-YC2743. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 3 Anti-SIGLEC15 Monoclonal Antibody (V3S-0522-YC2743) in ELISA

For research use only, not directly for clinical use.
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