| Description | This product is a human monoclonal antibody that reacts with TNFRSF10A. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | The extracellular region of human DR4 fused to the Fc region of human gamma- 1 heavy chain (DR4-Fc) |
| Epitope | In the ECD domain |
| Isotype | IgG1 lambda |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA |
| Application Notes | The antibody is recommended for detection of TNFRSF10A by ELISA assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | TNFRSF10A |
| Alternative Name | TNF Receptor Superfamily Member 10a; Tumor Necrosis Factor Receptor Superfamily, Member 10a; TNF-Related Apoptosis-Inducing Ligand Receptor 1; Death Receptor 4; TRAIL Receptor 1; TRAIL-R1; TRAILR1; APO2; |
| Gene ID | 8797 |
| UniProt | O00220 |
| Research Area | Cell Biology; Immunology |
