| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize TNF receptor superfamily member 4. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG1, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA; IHC; FC; IP; IF; FuncS |
| Application Notes | The antibody is recommended for detection of TNFRSF4 by ELISA, IHC, FC, IP, IF, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-TNFRSF4 Monoclonal Antibody (V3S-0522-YC1471) in SDS-PAGE
SDS-PAGE analysis of V3S-0522-YC1471 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 175-270 kDa.
Figure 1 Anti-TNFRSF4 Monoclonal Antibody (V3S-0522-YC1471) in SDS-PAGE
Figure 2 Anti-TNFRSF4 Monoclonal Antibody (V3S-0522-YC1471) in SEC-HPLC
The purity of V3S-0522-YC1471 was greater than 93% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.3
Detection: UV 280 nm
Injection: 25 µl
Figure 2 Anti-TNFRSF4 Monoclonal Antibody (V3S-0522-YC1471) in SEC-HPLC
Figure 3 Anti-TNFRSF4 Monoclonal Antibody (V3S-0522-YC1471) in WB
Lane 1: Non-Reducing Antigen (0.5 μg)
Lane 2: Reducing Antigen (0.5 μg)
Figure 3 Anti-TNFRSF4 Monoclonal Antibody (V3S-0522-YC1471) in WB
Figure 4 Anti-TNFRSF4 Monoclonal Antibody (V3S-0522-YC1471) in ELISA
ELISA analysis of V3S-0522-YC1471 was performed by coating with Recombinant Human TNFRSF4 Protein.
Figure 4 Anti-TNFRSF4 Monoclonal Antibody (V3S-0522-YC1471) in ELISA
