| Description | This product is a human monoclonal antibody that reacts with TNFRSF8. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Hodgkin's lymphoma cell line L428. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | Neut; ELISA; IF; IP; FuncS; FC; ICC |
| Application Notes | The antibody is recommended for detection of TNFRSF8 by Neut, ELISA, IF, IP, FuncS, FC, ICC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-Human TNFRSF8 Monoclonal Antibody (V3S-0622-YC5673) in SDS-PAGE
SDS-PAGE analysis of V3S-0622-YC5673 in reduced (lane 1, 2 μg) and non-reduced (lane 2, 2 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 25 kDa and 50 kDa, respectively. Non-reducing protein migrates about 150-200 kDa.
Figure 1 Anti-Human TNFRSF8 Monoclonal Antibody (V3S-0622-YC5673) in SDS-PAGE
Figure 2 Anti-Human TNFRSF8 Monoclonal Antibody (V3S-0622-YC5673) in SEC-HPLC
The purity of V3S-0622-YC5673 was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 2 Anti-Human TNFRSF8 Monoclonal Antibody (V3S-0622-YC5673) in SEC-HPLC
Figure 3 Anti-Human TNFRSF8 Monoclonal Antibody (V3S-0622-YC5673) in ELISA
ELISA analysis of V3S-0622-YC5673 was performed by coating with Recombinant Human CD30 Protein. Then blocked with BSA, and incubated with Anti-Human CD30 Antibody (V3S-0622-YC5673). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-Human TNFRSF8 Monoclonal Antibody (V3S-0622-YC5673) in ELISA
