Anti-TNFRSF9 (ECD domain) Neutralizing Antibody (V3S-0622-YC5675) (CAT#: V3S-0622-YC5675)

Figure 1 Anti-TNFRSF9 Monoclonal Antibody (V3S-0622-YC5675) in SDS-PAGE

SDS-PAGE analysis of V3S-0622-YC5675 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180 kDa.

Figure 2 Anti-TNFRSF9 Monoclonal Antibody (V3S-0622-YC5675) in SEC-HPLC

The purity of V3S-0622-YC5675 was greater than 94% as determined by SEC-HPLC.

Figure 3 Anti-TNFRSF9 Monoclonal Antibody (V3S-0622-YC5675) in WB

Western blot analysis of V3S-0622-YC5675 was performed by loading Recombinant Human CD137 Protein (His Tag) (1 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk.
Lane 1: Non-Reducing Antigen (1 μg)
Lane 2: Reducing Antigen (1 μg)

Figure 4 Anti-TNFRSF9 Monoclonal Antibody (V3S-0622-YC5675) in ELISA

ELISA analysis of V3S-0622-YC5675 was performed by coating with Recombinant Human CD137 Protein (His Tag).