Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1343) (CAT#: V3S-0522-YC1343)

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  • fig1
    Figure 1 Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1343) in SDS-PAGE.
  • fig1
    Figure 2 Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1343) in SEC-HPLC.
  • fig1
    Figure 3 Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1343) in Western Blot.
  • fig1
    Figure 4 Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1343) in ELISA.

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Tumor necrosis factor superfamily member 15. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Immunogen Human recombinant TL1A
Isotype IgG2a, κ

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application ELISA; FC; Block
Application Notes The antibody is recommended for detection of TNFSF15 by ELISA, FC, Block assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target TNFSF15
Alternative Name TNFSF15; Tumor necrosis factor superfamily member 15; TL1; TL1A; VEGI; VEGI192A; tumor necrosis factor ligand superfamily member 15; TNF superfamily ligand TL1A; TNF ligand-related molecule 1; vascular endothelial cell growth inhibitor; vascular endothelial growth inhibitor-192A
Gene ID 9966
UniProt O95150
Research Area Cardiovascular
Related Disease Asthma, inflammatory bowel disease

Tested Data

SDS-PAGE

Figure 1 Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1343) in SDS-PAGE.

SDS-PAGE analysis of V3S-0522-YC1343 in reduced (Lane 1) and non-reduced (Lane 2) conditions.
The purity of V3S-0522-YC1343 was greater than 95% as determined by SDS-PAGE.

Figure 1 Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1343) in SDS-PAGE.

HPLC

Figure 2 Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1343) in SEC-HPLC.

The purity of V3S-0522-YC1343 was 99.0172% as determined by SEC-HPLC.

Figure 2 Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1343) in SEC-HPLC.

WB

Figure 3 Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1343) in Western Blot.

Western blot analysis of V3S-0522-YC1343 was performed by loading recombinant human TNFSF15 protein.

V3S-0522-YC1343 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-mouse IgG

Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 3 Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1343) in Western Blot.

ELISA

Figure 4 Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1343) in ELISA.

ELISA analysis of V3S-0522-YC1343 was performed by coating with recombinant human TNFSF15 protein. Then blocked with BSA and incubated with antibody V3S-0522-YC1343. The goat anti-mouse IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 4 Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1343) in ELISA.

For research use only, not directly for clinical use.
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