Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1354) (CAT#: V3S-0522-YC1354)

Figure 1 Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1354) in SDS-PAGE.

SDS-PAGE analysis of V3S-0522-YC1354 in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

The purity of V3S-0522-YC1354 was greater than 95% as determined by SDS-PAGE.

Figure 2 Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1354) in SEC-HPLC.

The purity of V3S-0522-YC1354 was 95.9931% as determined by SEC-HPLC.

Figure 3 Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1354) in Western Blot.

Western blot analysis of V3S-0522-YC1354 was performed by loading recombinant human TNFSF15 protein protein.

V3S-0522-YC1354 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG

Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 4 Anti-TNFSF15 Neutralizing Antibody (V3S-0522-YC1354) in ELISA.

ELISA analysis of V3S-0522-YC1354 was performed by coating with recombinant human TNFSF15 protein. Then blocked with BSA and incubated with antibody V3S-0522-YC135. The goat anti-human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.