ANGPT2 (aa 255-495) Specific Recomb™ Antibody (V3S-0324-FY5), Human IgG1 kappa (CAT#: V3S-0324-FY5)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This is a human IgG1, κ monoclonal antibody that is specific binding to Angiopoietin-2.The antibody can bind to amino acids 255 to 495 of human Angiopoietin-2. It also cross-reacts with mouse Angiopoietin-2 and the affinity is 0.12 nM (human ang-2), 0.15 nM (mouse ang-2) respectively.
Clonality Monoclonal
Host Species Human
Target Species Human, Mouse
Epitope Amino acids 255-495
IC50 0.08 nM for human ang-2, 0.05 nM for mouse ang-2, 114.65 nM for human ang-1
EC50 0.005 nM for human ang-2, 0.01 nM for mouse ang-2, 30 nM for human ang-1
Affinity 0.12 nM for human ang-2, 0.15 nM for mouse ang-2
Isotype Human IgG1, kappa

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within a week. For longer storage, aliquot and store at -20°C.

Applications

Application ELISA

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target ANGPT2
Alternative Name Angiopoietin 2; ANG-2; Angiopoietin-2B; Angiopoietin-2a; Angiopoietin-2; Tie2-Ligand; AGPT2; ANG2
Gene ID 285
UniProt O15123
Related pathway MIF Mediated Glucocorticoid Regulation and TGF-Beta Pathway
Research Area Signal Pathway
Related Disease Cancer
For research use only, not directly for clinical use.
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