| Description | This mouse anti-BSG mAb (clone M6-1B9) recognizes human BSC (CD147). M6-1B9 recognizes both native (glycosylated) and recombinant (non-glycosylated) CD147 molecules. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Recombinant CD147-biotin carboxyl carrier protein (BCCP) |
| ED50 | 3.81 mg/kg |
| Function | Isoform 1: Essential for normal retinal maturation and development. Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed: 25957687). In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed: 25957687). May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes (PubMed: 21620857). Isoform 2 Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells (PubMed: 11943775, PubMed: 11688976). Plays an important role in targeting monocarboxylate transporters SLC16A1/GLUT1, SLC16A11 and SLC16A12 to the plasma membrane (PubMed: 17127621, PubMed: 21778275, PubMed: 28666119). Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling (PubMed: 25825981). Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA (isoform VEGF-165 and VEGF-121) and KDR/VEGFR2 in endothelial cells (PubMed: 19837976). Plays a key role in regulating tumor growth, invasion, metastasis and neoangiogenesis by stimulating the production and release of extracellular matrix metalloproteinases and KDR/VEGFR2 by both tumor cells and stromal cells (fibroblasts and endothelial cells) (PubMed: 12553375, PubMed: 11992541, PubMed: 15833850). |
| Isotype | Mouse IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB; FC; ICC |
| Application Notes | The specificity of the antibody toward CD147 was analyzed by indirect ELISA using a recombinant CD147-BCCP as a target. The antibody has been tested in use for flow cytometry on U937 and 293A cells. The colocalization of this clone and CD147 was analyzed by Immunocytochemical analysis in transfected 293A cells. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Post Translational Modification | Isoform 2: N-glycosylated. Isoform 3: N-glycosylated. Isoform 4: N-glycosylated. |
