| Description | This mouse anti-BSG mAb (clone M6-1B9) recognizes human BSC (CD147). M6-1B9 recognizes both native (glycosylated) and recombinant (non-glycosylated) CD147 molecules. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Recombinant CD147-biotin carboxyl carrier protein (BCCP) |
| ED50 | 3.81 mg/kg |
| Function | Isoform 1: Essential for normal retinal maturation and development. Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed: 25957687). In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed: 25957687). May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes (PubMed: 21620857). Isoform 2 Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells (PubMed: 11943775, PubMed: 11688976). Plays an important role in targeting monocarboxylate transporters SLC16A1/GLUT1, SLC16A11 and SLC16A12 to the plasma membrane (PubMed: 17127621, PubMed: 21778275, PubMed: 28666119). Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling (PubMed: 25825981). Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA (isoform VEGF-165 and VEGF-121) and KDR/VEGFR2 in endothelial cells (PubMed: 19837976). Plays a key role in regulating tumor growth, invasion, metastasis and neoangiogenesis by stimulating the production and release of extracellular matrix metalloproteinases and KDR/VEGFR2 by both tumor cells and stromal cells (fibroblasts and endothelial cells) (PubMed: 12553375, PubMed: 11992541, PubMed: 15833850). |
| Isotype | Mouse IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB; FC; ICC |
| Application Notes | The specificity of scFv M6-1B9 toward CD147 was analyzed by indirect ELISA using a recombinant CD147-BCCP as a target. The result of Western Blotting showed the expression of Fab M6-1B9 and scFv M6-1B9 on phage particles. The scFv M6-1B9 has been tested in use for Flow Cytometry on U937 and 293A cells. The colocalization of scFv-M6-1B9 intrabody and CD147 was analyzed by Immunocytochemical analysis in transfected 293A cells. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Post Translational Modification | Isoform 2: N-glycosylated. Isoform 3: N-glycosylated. Isoform 4: N-glycosylated. |
