| Description | Compared to its humanized mAb, the affinity of this antibody was improved. The equilibrium dissociation constant (KD) of the mAb to human CD19 is 0.189 μM as measured by SPR. This antibody was designed to be devoid of deamidation hotspots in sequence (LCDR1 N27d and N28 hotspots). |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Affinity | KD = 0.189 μM |
| Isotype | Human IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; SPR |
| Application Notes | This antibody can be used for detection of human CD19 by ELISA. The Affinity to human CD19 was measured by SPR at 25 °C. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Post Translational Modification | Phosphorylated on tyrosine following B-cell activation (PubMed:7684160, PubMed:7687539, PubMed:10706702, PubMed:12387743). Phosphorylated on tyrosine residues by LYN (PubMed:7687428). Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels (By similarity). |
