| Description | Compared to its parental humanized mAb (clone 8B8), the affinity of the antibody 8B8-5H09 was improved. The equilibrium dissociation constant (KD) of 8B8-5H09 to human CD19 is 0.189 μM as measured by SPR. This antibody was designed to be devoid of deamidation hotspots in sequence (LCDR1 N27d and N28 hotspots). |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Affinity | KD = 0.189 μM |
| Isotype | Human IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; SPR |
| Application Notes | This antibody can be used for detection of human CD19 by ELISA. The Affinity to human CD19 of 8B8-5H09 was measured by SPR at 25 °C. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Post Translational Modification | Phosphorylated on tyrosine following B-cell activation (PubMed:7684160, PubMed:7687539, PubMed:10706702, PubMed:12387743). Phosphorylated on tyrosine residues by LYN (PubMed:7687428). Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels (By similarity). |
