| Description | This recombinant anti-EBOV VP35 antibody (clone E1) recognizes the Ebola filovirus (EBOV) VP35 protein. E1 recognized recombinant EBOV VP35 in ELISA but showed no reactivity in denaturing conditions of WB, suggesting that E1 probably recognize conformational epitopes. E1 has a significant inhibition effect on the IFN-β-induced RIG-I signaling cascade mediated by VP35 in A549 cells. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Ebola filovirus (EBOV) |
| Epitope | Conformational epitope |
| Function | Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling. |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Inhib; FuncS |
| Application Notes | This anti-EBOV VP35 antibody is able to react with recombinant VP35 protein analyzed by ELISA. E1 has a significant inhibition effect on the VP35- mediated IFN-β-induced RIG-I signaling cascade in A549 cells. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | EBOV VP35 |
| Alternative Name | VP35; Polymerase complex protein |
| UniProt | X5GXU1 |
| Research Area | Microbiology; Infectious Disease |
| Related Disease | Ebola hemorrhagic fever |
