| Description | This human anti-EBOV VP35 antibody (clone A10) recognizes the Ebola filovirus (EBOV) VP35 protein. The results of ELISA and WB showd that A10 probably recognized a linear epitope. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Ebola filovirus (EBOV) |
| Epitope | A sequential epitope |
| Function | Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling. |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB |
| Application Notes | The results of ELISA and Western Blot showed that this anti-EBOV VP35 antibody is able to react with recombinant VP35 protein. In cell assay, the mAb showed no ability to counteract the inhibition of IFN-β induction. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | EBOV VP35 |
| Alternative Name | VP35; Polymerase complex protein |
| UniProt | X5GXU1 |
| Research Area | Microbiology; Infectious Disease |
| Related Disease | Ebola hemorrhagic fever |
