| Description | This is a mouse antibody that binds to human TIM-3. The antibody binds to hTIM3-FC with a value of 1.388, which was measured by absorbance at 450 nm. It binds strongly to the consecutive C'C″ and DE loops, and mildly to the CC' loop. The antibody showed functional activity related to pro-inflammatory cytokines. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Human TIM-3-Fc protein |
| Epitope | It binds strongly to the consecutive C'C″ and DE loops, and mildly to the CC' loop. |
| Isotype | Mouse IgG |
| Expression Species | HEK293F or CHO |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; FuncS |
| Application Notes | Functional assay (FuncS): 25 μg/mL |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HAVCR2 |
| Alternative Name | Hepatitis A Virus Cellular Receptor 2; T-Cell Immunoglobulin And Mucin Domain-Containing Protein 3; T-Cell Immunoglobulin Mucin Family Member 3; T-Cell Immunoglobulin Mucin Receptor 3; T-Cell Membrane Protein 3; HAVcr-2; TIMD-3; Tim-3 |
| Gene ID | 84868 |
| UniProt | Q8TDQ0 |
| Related pathway | Wnt/beta-catenin pathway |
| Research Area | Autophagy; Signal Pathway |
| Related Disease | Autoimmune disease |
