| Description | This product is a neutralizing antibody that binds to Human immunodeficiency virus-1. The antibody is a recombinant monkey mAb. DH727.2 bound well to clade A and clade C V3 peptides-consistent with its neutralizing profile. DH727.2 showed similarity to other V3 peptide antibodies in that its paratope exhibited a defined cleft between the heavy and light chains into which the β-hairpin structure of the V3 crown bound. |
| Clonality | Monoclonal |
| Host Species | Monkey |
| Target Species | HIV1 |
| Epitope | Within the V3 loop, Gln315 was strongly favored by DH727.2. |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA |
| Application Notes | In total 2 μg/mL of protein in sodium bicarbonate buffer was incubated in sealed Nunc-absorp plates overnight at 4°C. Unbound protein was washed away and the plates were blocked with SuperBlock for 1 h. Serially dilution of monoclonal antibodies were added to the plate for 90 min. Binding antibodies were detected with 1:30,000 dilution of HRP labeled anti_x0002_IgG Fc antibodies. HRP was detected with 3,3',5,5'-Tetramethylbenzidine. Binding titers were analyzed as area-under_x0002_curve of the log-transformed concentrations. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HIV1 |
| Alternative Name | Human immunodeficiencyvirus-1 |
| Research Area | Infectious Disease |
| Related Disease | Acquired Immune Deficiency Syndrome |
