| Description | This product is a neutralizing antibody that binds to Human papillomavirus 58. The antibody is a recombinant mouse mAb. NAb A4B4 recognizes a linear epitope, as indicated by Western blotting, which may be derived from one of two distinct loops that constitute a conformational epitope revealed by the cryo-EM analysis. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | HPV |
| Immunogen | Purified HPV58 virus-like particles (VLPs) |
| Epitope | fringe binding (linear epitope) |
| Isotype | IgG2a |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA |
| Application Notes | The 96-well plates were coated with antigens (300 ng/well) overnight at 4°C and then blocked with blocking buffer for 1 h at 37°C. HPV58 type-specific antibodies were added at the indicated concentrations for 45 min at 37°C. Subsequently, the plates were washed with PBST and then incubated with HRP-conjugated secondary antibodies (goat anti_x0002_mouse IgG) for an additional 45 min. The assays were developed with tetramethylbenzidine, and the reaction was terminated. Absorbance was measured at 450 nm with a reference wave_x0002_length of 620 nm using a Multiskan Spectrum. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HPV58 |
| Alternative Name | Human papillomavirus type 58 |
| Research Area | Infectious Disease |
| Related Disease | Thymion; Cervical cancer |
