PDCD1 (ECD domain) Specific Recomb™ Antibody (V3S-0224-CJ13), Mouse IgG (CAT#: V3S-0224-CJ13)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target
Virus Background

Summary

Description Antibody 6F5H1L1 is a humanized mAb specifically binding to human PDCD1 (programmed cell death 1), well-known as PD-1. This anti-PDCD1 antibody can effectively block the binding of PD-1 and its ligand PD-L1. The parental murine monoclonal antibody (6F5) was generated in BALB/c mice by immunization with recombinant PD-1(ECD)-TEV-mFc. The binding activities of this antibody were measured by ELISA.
Clonality Monoclonal
Host Species Human
Target Species Human
Immunogen Recombinant PD-1 extracellular domain-TEV-mouse Fc fragment
Epitope The extracellular domain
EC50 0.118 nM (indirect ELISA; immunogen: PD-1-mFc, 0.5 μg/mL)
0.979 nM (competitive ELISA; immunogen: PD-1-hFc, 0.5 μg/mL)
Isotype Humanized IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within a week. For longer storage, aliquot and store at -20°C.

Applications

Application ELISA
Application Notes The binding activities of this antibody were measured by ELISA. Competitive ELISA was used to determine the ability of humanized antibody 6F5H1L1 to compete with PD-L1 (0.3 μg/mL) for binding the antigen PD-1 (0.5 μg/mL).

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target PDCD1
Alternative Name CD279; PD-1; PD1; SLEB2; hPD-1; hPD-l; hSLE1
Gene ID 5133
UniProt Q15116
Related pathway Infectious disease; SARS-CoV-2 Infection
Research Area Epigenetics
Related Disease Cancer; Systemic Lupus Erythematosus; Multiple Sclerosis

Virus Background

Post Translational Modification Ubiquitinated at Lys-233 by the SCF(FBXO38) complex, leading to its proteasomal degradation (PubMed:30487606).
Ubiquitinated via 'Lys-48'-linked polyubiquitin chains (PubMed:30487606).
Tyrosine phosphorylated at Tyr-223 (within ITIM motif) and Tyr-248 (ITSM motif) upon ligand binding. Phosphorylation at Tyr-248 promotes the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta.
N-glycosylation at Asn-58 contains at least two N-acetylglucosamine units and one fucose (PubMed:28165004).
For research use only, not directly for clinical use.
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